Infect Dis Poverty (2021) 10:15. Real-time polymerase chain reaction (PCR) is a sensitive and specific method for diagnosing schistoso- miasis. However, this method should be performed in a laboratory, usually located distant from the sample collection site. Therefore, it is important to have fas ... t sampling preservation methods, which allow simple transport prior to DNA extraction and amplification. The aim of this study was to verify if blood samples applied to filter paper are suitable for analysis of Schistosoma mansoni DNA by real-time PCR. more

Acta Trop. 2020 Mar:203:105289. doi: 10.1016/j.actatropica.2019.105289 The role of malacological surveys to identify potential transmission sites for schistosomiasis control in this era of mass drug administration have received little attention. In that context, the present study was conducted to ... determine the abundance, identity and disease transmission potential of intermediate host snails for intestinal schistosomiasis on Ijinga Island, north-western Tanzania. more

Infectious Diseases of Poverty (2020) 9:74 To detect acute schistosomiasis, low-intensity infections, or to verify the success of treatment with praziquantel, highly sensitive test methods are required. The aim of this study was therefore to demonstrate the performance of Schistosoma mansoni spec ... ific DNA detection in serum and urine using real-time polymerase chain reaction (PCR) in an endemic area before and after treatment. more

PLoS ONE 13(8): e0202499. https://doi.org/10.1371/journal.pone.0202499 This was a school-based cross-sectional study conducted in 2015 among 305 school children aged 7–16 years from two primary schools located in Ilemela and Magu Districts, north-western Tanzania. Single stool and urine samples w ... ere collected from each participant and examined for the presence of Schistosoma mansoni eggs, parasite antigen, and parasite DNA using KK thick smears, POC-CCA tests, and real-time PCR, respectively. more