The latest update (28 January 2021) includes the following addition and revision: biosafety aspects for working with antigen-detecting rapid diagnostic test; handling new variants of SARS-CoV-2 in the laboratory; updated assay decontamination before disposal; personal protective equipment (PPE) for specimen collection; addressing chemical hazards and their safe disposal; and the fourth edition of the WHO Laboratory Biosafety Manual (LBM4) is now available and the terminology in this guidance was aligned with the LBM4.