The Ebola Virus Disease (EVD) Secondary and Tertiary Screening Tool provides guidance for healthcare workers on conducting more detailed assessments of individuals who have already been identified as potential Ebola suspects during primary screening. The tool outlines procedures for evaluating symptoms, exposure history, and risk factors to determine whether a person meets the criteria for a suspected Ebola case. Its purpose is to support accurate case identification, ensure timely isolation and referral of suspected patients, and strengthen infection prevention and control measures within healthcare facilities. The tool was developed to enhance Uganda’s preparedness and response capacity during Ebola outbreaks, particularly the 2022 Sudan Ebola Virus Disease outbreak.