By end of March 2022: 42 COVID-19 detection laboratories were assessed in the 1st Quarter 2022. 23 (55%) Labs are reported fully functional, while 19 (45%) requires assistance to fully operate (i.e., medical supplies, equipment, maintenance, and staffing). 34 (89%) labs have RT-PCR machines, while 4 (11%) doesn’t have. Most laboratories operate 1 shift of 8 hours 13 (34%), followed by 2 shifts of 16 hours 11 (29%). Over 1000 testing capacity per day is reported in Tripoli Biotechnical Centre Lab (5,000), Azzawya NCDC Lab (2,500), Tripoli NCDC public health lab (1500), Misrata NCDC Lab (1,300), and Misrata Medical centre Lab (1,300). The reported needs ranked per priority are equipment, consumables, and staff training; consequently.