For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of Trypanosoma cruzi in human serum is affected by various influences including the choice of th...e nucleic acid extraction assay. In this study, three nucleic acid extraction assays were compared regarding their influence on the sensitivity of a T. cruzi-specific real-time PCR with 62 reference sera containing T. cruzi target DNA (deoxyribonucleotide acid). More than 95% of the positive sera were correctly identified after all three nucleic acid extraction strategies with a detection rate ranging from 96.8% (60/62) for the worst assay to 100% (62/62) for the best one. A matched pairs analysis for the comparison of the cycle threshold (Ct) values obtained with the 59 reference samples with positive real-time PCR results after all three nucleic acid extraction schemes indicated differences in a range of about 3 Ct steps. Summarized, all three compared nucleic acid extraction schemes were basically suitable for T. cruzi-specific PCR from serum with some minor differences. However, in the case of low quantities of circulating parasite DNA in the serum of a patient with Chagas disease, even minor effects can make a difference in the individual diagnosis.
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LAM test as a diagnostic aid in patients with HIV and suspected tuberculosis: technical guidelines
Consensus -based guidelines for diagnosis, prevention and treatment of tuberculosis in children and adolescents
La técnica diagnóstica ampliamente usada a nivel mundial corresponde a la Prueba de Tuberculina o test de Mantoux, la que a través de la inyección de antígenos de Micobacterium tuberculosis permite medir la reacción del individuo y estimar su probabilidad de infección. El presente manual tie...ne como objetivo describir la técnica para la realización del examen, además de ofrecer lineamientos para su uso programático en Chile.
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You can download all diagnostic reference resources from the website: http://www.cdc.gov/dpdx/az.html
In this book, the authors present a dozen state of the art developments for ultrasound imaging, for example, hardware implementation, transducer, beamforming, signal prcessing, measurement of elasticity and diagnosis. Read/download chapter by chapter
Part of: Clinical guidelines - Diagnosis and treatment manual Chapter 6: Parasitic diseases
Guidelines for diagnosis, acute care and post-COVID-19 follow-up for children and adolescents
Protocol for laboratory diagnosis of COVID_19, in the framework of the health emergency
Guidelines for the diagnosis and treatment of COVID-19 in intensive care units
Evaluation and Diagnosis of Penicillin Allergy for Healthcare Professionals
Adapted from CURRENT Medical Diagnosis & Treatment 2010
Guidelines for Diagnosis and Management of Lung Cancer in Indonesia
Despite the development of point-of-care diagnostic tests for syphilis, chlamydia, gonorrhoea, and trichomoniasis, none comply with all WHO criteria. This analysis overviews landscape analyses of point-of-care diagnostic technologies for Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vagi...nalis and syphilis, available and in the pipeline. The target audience for the target product profiles is broad and includes clinicians, researchers working on diagnostics, laboratory experts, including, microbiologists and virologists, public health experts, epidemiologists, developers, and representatives for manufactures, including biotech engineers, policy-and decision-makers as well as representatives from regulatory bodies and agencies, donor agencies and international organizations.
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Barriers to the prompt and effective diagnosis and treatment of malaria exist at both the community and health facility level. Household surveys measure malaria case management at the population level with standard indicators that assess treatment-seeking behavior, access to diagnostic testing, and ...access to appropriate treatment. Performance on these indicators varies widely from country to country. Among countries with Demographic and Health Surveys (DHS) or Malaria Indicator Surveys (MIS) completed between 2014 and 2016, advice and treatment was sought for a median of 47% of children under age 5 with fever.
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Pulmonary tuberculosis predictors and rapid molecular diagnosis
Preditores de tuberculose pulmonar e experiência com o diagnóstico molecular rápido
Molecular methods for antimicrobial resistance (AMR)diagnostics to enhance the Global Antimicrobial Resistance Surveillance System
The internationally recognized criteria for diagnosis of neurocysticercosis include a requirement for neuroimaging techniques, such as computerized tomography (CT) and/or magnetic resonance imaging (MRI), ideally supported by serology. These facilities are not available in all settings, especially i...n rural areas of low-income countries, making it difficult to identify and treat patients. Additionally, there is controversy about the role, type and duration of anthelmintic, antiinflammatory and antiepileptic drug (AED) treatments for different forms of neurocysticercosis.
These guidelines were developed to assist health-care providers in appropriate, evidence-based management of parenchymal neurocysticercosis. The guidelines do not address other forms of neurocysticercosis and do not include management of extraparenchymal disease (including cysticerci in the cerebral ventricles or subarachnoid space). The aim of the guidance is to improve decision-making to ensure appropriate patient care and to avoid misdiagnoses and inappropriate treatment of patients with neurocysticercosis.
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Early breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up
The key areas covered are diagnosis, imaging, pathology, surgery, rehabilitation, palliative care and survivorship. It emphasizes a multi-disciplinary team approach which is paramount for quality cancer care. The specific cancers covered are breast, central nervous system, gastroint...estinal, gynecological, head and neck, hematological, Kaposi’s sarcoma, lung, prostate and pediatric cancers. They also complement the National Guidelines for Cancer Management in Kenya released in 2013.
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