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Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania

Fuss, A.; H. D. Mazigo and A. Mueller (2021) CC

Infect Dis Poverty (2021) 10:15. Real-time polymerase chain reaction (PCR) is a sensitive and specific method for diagnosing schistoso- miasis. However, this method should be performed in a laboratory, usually located distant from the sample collection site. Therefore, it is important to have fas... more

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Establishment of PCR laboratory in developing countries, 2nd ed.
recommended

World Health Organization ( WHO), Regional Office for South-East-Asia (2016) C_WHO

Polymerase Chain Reaction (‎PCR)‎ has significantly helped in early diagnosis and commencement of specific interventions for diseases control. It also plays a critical role in understanding the disease epidemiology and unraveling the transmission dynamics of the disease. This manual intends to p... more

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Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity of Trypanosoma cruzi-Specific Real-Time PCR

Kann, S.; Zabala-Monterroza, W.; García, C. et al. Multidisciplinary Digital Publishing Institute MDPI (2022) CC

For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of Trypanosoma cruzi in human serum is affected by various influences including the choice of th... more

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Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment

Fuss, A.; H. D. Mazigo and A. Mueller (2020) CC

Infectious Diseases of Poverty (2020) 9:74 To detect acute schistosomiasis, low-intensity infections, or to verify the success of treatment with praziquantel, highly sensitive test methods are required. The aim of this study was therefore to demonstrate the performance of Schistosoma mansoni spec... more

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Fourth annual meeting of the network of Buruli ulcer PCR laboratories in the WHO African Region: Mundi complex, Yaoundé, 24-26 October 2022

World Health Organization WHO (2024) C_WHO

n October 2019, WHO convened the first meeting of the Buruli ulcer laboratory network (BU-LABNET) in Yaoundé, Cameroon, bringing together 11 laboratories from nine countries at the Pasteur Centre of Cameroon (CPC), the network’s Coordinating Centre. The network was formally established at th... more

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Chagas Disease: Detection of Trypanosoma cruzi by a New, High-Specific Real Time PCR

Kann, S.; Kunz, M.; Hansen, J.; et al. (2020) CC

J Clin Med . 2020 May 18;9(5):1517. doi: 10.3390/jcm9051517. Chagas disease (CD) is a major burden in Latin America, expanding also to non-endemic countries. A gold standard to detect the CD causing pathogen Trypanosoma cruzi is currently not available. Existing real time polymerase chain reaction... more

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Monitoring and Evaluation of COVID-19 Rapid Antigen Diagnostic Test Rollout in Africa
recommended

African Union; Africa CDC Centres for Disease Control and Prevention (2021) C1

Limited coverage of laboratory services and long turnaround times from real-time reverse transcription-polymerase chain reaction (rRT-PCR) for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has been insufficient to meet the demands in many African countries in response... more

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Marburg and Ebola Virus Infections

MSD Manual (2023) CC2

Marburg and Ebola are filoviruses that cause hemorrhage, multiple organ failure, and high mortality rates. Diagnosis is with enzyme-linked immunosorbent assay, polymerase chain reaction (PCR), or electron microscopy. Treatment is supportive. Strict isolation and quarantine measures are necessary to ... more

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Laboratory testing for the monkeypox virus
recommended

World Health Organization (WHO) (2022) C_WHO

Any individual that meets the suspected case definition of monkeypox should be offered testing in appropriately equipped laboratories by staff trained in the relevant technical and safety procedures. Confirmation of monkeypox virus infection is based on nucleic acid amplification testing (NAAT), usi... more

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Disease Outbreak News Marburg virus disease - Rwanda

World Health Organisation (WHO) (2024) C_WHO

On 27 September 2024, the Rwanda Ministry of Health announced the confirmation of Marburg virus disease (MVD). Blood samples taken from people showing symptoms were tested by real-time reverse transcription polymerase chain reaction (RT-PCR) at the National Reference Laboratory of the Rwanda Biomedi... more

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History, epidemiology and diagnostics of dengue in the American and Brazilian contexts: a review

Souza Salles, T.; T. da Encarnação Sá-Guimarães, E. Seam Lima de Alvarenga et al. (2018) CC

Parasites & Vectors volume 11, Article number: 264 (2018) Dengue creates a staggering epidemiological and economic burden for endemic countries. Without a specific therapy and with a commercial vaccine that presents some problems relative to its full effectiveness, initiatives to improve vector... more

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Utilisation de l’imagerie thoracique en cas de COVID-19
recommended

Organisation Mondiale de la Santé OMS (2020) C_WHO

Guide de recommandations rapides 11 juin 2020 Depuis son identification en Chine en décembre 2019, le nouveau coronavirus responsable de la COVID‐19 s’est rapidement propagé pour causer une pandémie. Cette maladie se manifeste par des symptômes respiratoires non spécifiques de gravité var... more

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Mpox Testing Strategy - November 2024

Africa Center for Disease Control and Prevention (2024) CC

Mpox is an emerging zoonotic disease caused by the mpox virus, a member of the Orthopoxvirus genus closely related to the variola virus that causes smallpox. Mpox was first discovered in 1958 when outbreaks of a pox-like disease occurred in monkeys kept for research. The first human case was recorde...d in 1970 in the Democratic Republic of the Congo (DRC) during a period of intensified effort to eliminate smallpox and since then the infection has been reported in a number of African countries. Mpox can spread in humans through close contact, usually skin-to-skin contact, including sexual contact, with an infected person or animal, as well as with materials contaminated with the virus such as clothing, beddings and towels, and respiratory droplets in prolonged face to face contact. People remain infectious from the onset of symptoms until all the lesions have scabbed and healed. The virus may spread from infected animals through handling infected meat or through bites or scratches. Diagnosis is confirmed by polymerase chain reaction (PCR) testing of material from a lesion for the virus’s DNA. Two separate clades of the mpox virus are currently circulating in Africa: Clade I, which includes subclades Ia and Ib, and Clade II, comprising subclades IIa and IIb. Clade Ia and Clade Ib have been associated with ongoing human-to-human transmission and are presently responsible for outbreaks in the Democratic Republic of the Congo (DRC), while Clade Ib is also contributing to outbreaks in Burundi and other countries. In 2022‒2023 mpox caused a global outbreak in over 110 countries, most of which had no previous history of the disease, primarily driven by human-to-human transmission of clade II through sexual contact. In just over a year, over 90,000 cases and 150 deaths were reported to the WHO. For the second time since 2022, mpox has been declared a global health emergency as the virus spreads rapidly across the African continent. On 13 Aug 2024, Africa CDC declared the ongoing mpox outbreak a Public Health Emergency of Continental Security (PHECS), marking the first such declaration by the agency since its inception in 2017.7 This declaration empowered the Africa CDC to lead and coordinate responses to the mpox outbreak across affected African countries. On August 14, 2024, the WHO declared the resurgence of mpox a Public Health Emergency of International Concern (PHEIC) emphasizing the need for coordinated international response. As of August 2024, Mpox has expanded beyond its traditional endemic regions, with new cases reported in countries including Sweden, Thailand, the Philippines, and Pakistan. Sweden has confirmed its first case of Clade 1 variant, which has been rapidly spreading in Africa, particularly in DRC. The emergence of this new variant raises concerns about its potential for higher lethality and transmission rates outside Africa. more

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Diagnostic detection of 2019-nCoV by real-time RT-PCR

Corman, V.; T. Bleicker; S. Brünink; et al. Charité Berlin (2020) C1

Protocol and preliminary evaluationas of Jan 17, 2020

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Collection of Dried Blood Spots (DBS) from Infants for PCR Testing

Centers for Disease Control and Prevention (CDC) (2018) C1

This clinical job aid provides health care workers with information on how to collect specimens for early infant diagnosis on dried blood spots, as well as drying and packaging for transport.

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Mandat Réseau de laboratoires et nouveau modèle de programme d’évaluation externe de la qualité des tests de diagnostic par PCR de l’ulcère de Buruli dans la Région africaine de l’OMS

Organisation Mondiale de la Santé OMS (2020) C1

Au total, 18 laboratoires de 13 pays ont participé aux quatre cycles d'AQE : 10 laboratoires de huit pays africains endémiques, dont quatre ont participé aux quatre cycles et trois à trois cycles. Les résultats globaux ont montré que la performance médiane de ces laboratoires s'est amélioré... more

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Coronavirus (COVID-19)genesig®Real-Time PCR assay

Primerdesign LTD (2020) C1

Instruction for Use

External Link

Diagnostik zum Nachweis von COVID-19
recommended

AMBOSS (2021) C2

Im Kapitel zu Diagnostik zum Nachweis von COVID-19 bekommen Sie ausführlich die verschiedenen Testmethoden erklärt, es können Videos zu PCR, RAchenabstrich, etc. aufgerufen werden

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FIND COVID-19 Diagnostics Resource Centre
recommended

FIND (2020) CC

FIND is collating an overview of SARS-CoV-2 tests that are commercially available or in development for the diagnosis of COVID-19. We do not guarantee that this is a comprehensive list, since the information below has been submitted voluntarily by test suppliers and is not independently verified. Pl... more

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SARS-CoV-2 diagnostic pipeline

Find (2020) C_WHO

Many in-house and commercial assays that detect the COVID-19 virus have been developed or are currently under development. Many of these molecular assays are currently being validated in partner laboratories. An overview of assays that have applied to FIND for participation in their assay assessment... more

Establishment of PCR laboratory in developing countries, 2nd ed. recommended

Monitoring and Evaluation of COVID-19 Rapid Antigen Diagnostic Test Rollout in Africa recommended

Laboratory testing for the monkeypox virus recommended

Utilisation de l’imagerie thoracique en cas de COVID-19 recommended

Diagnostik zum Nachweis von COVID-19 recommended

FIND COVID-19 Diagnostics Resource Centre recommended

Establishment of PCR laboratory in developing countries, 2nd ed.
recommended

Monitoring and Evaluation of COVID-19 Rapid Antigen Diagnostic Test Rollout in Africa
recommended

Laboratory testing for the monkeypox virus
recommended

Utilisation de l’imagerie thoracique en cas de COVID-19
recommended

Diagnostik zum Nachweis von COVID-19
recommended

FIND COVID-19 Diagnostics Resource Centre
recommended