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Mpox Molecular Diagnostic Tests(RT-PCR)

Africa Center for Disease Control and Prevention (2024) CC
On 13 August 2024, Africa Centres for Disease Control and Prevention (Africa CDC) declared the ongoing Mpox outbreak a Public Health Emergency of Continental Security (PHECS). This was followed the next day by the World Health Organization (WHO), which extended the alert internationally as a public ... more
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Updated coronavirus PCR and antigen testing criteria during the third wave

Wesern Cape Government, South Africa; PACK (2021) CC
25 June 2021
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Coronavirus (COVID-19)genesig®Real-Time PCR assay

Primerdesign LTD (2020) C1
Instruction for Use
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Diagnostic detection of 2019-nCoV by real-time RT-PCR

Corman, V.; T. Bleicker; S. Brünink; et al. Charité Berlin (2020) C1
Protocol and preliminary evaluationas of Jan 17, 2020
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Establishment of PCR laboratory in developing countries, 2nd ed.
recommended

World Health Organization ( WHO), Regional Office for South-East-Asia (2016) C_WHO
Polymerase Chain Reaction (‎PCR)‎ has significantly helped in early diagnosis and commencement of specific interventions for diseases control. It also plays a critical role in understanding the disease epidemiology and unraveling the transmission dynamics of the disease. This manual intends to p... more
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Zika virus PCR-standard

Nitsche, A. Robert-Koch-Institut (2016) CC
Update 19 May 2016
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First meeting of the network on Buruli ulcer PCR laboratories in the WHO African Region Centre Pasteur du Cameroon, Yaoundé, 21–24 October 2019

World Health Organization WHO (2020) CC
Research to develop point-of-care tests is in progress. Treatment of Buruli ulcer comprises 8 weeks of combined antibiotics (rifampicin and clarithromycin). Complementary therapies such as wound care, skin graft and prevention of disability are needed in some cases to ensure full recovery. The targ... more
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Première réunion du réseau de laboratoires PCR pour l’ulcère de buruli dans la région africaine de l’OMS, Centre Pasteur du Cameroun, Yaoundé, 21–24 octobre 2019

Organisation Mondiale de la Santé OMS (2020) CC
L’ulcère de Buruli, une infection causée par Mycobacterium ulcerans, touche plus de 33 pays dans le monde, mais un peu moins de la moitié seulement de ces pays communiquent régulièrement des données sur la maladie à l’OMS.
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Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity of Trypanosoma cruzi-Specific Real-Time PCR

Kann, S.; Zabala-Monterroza, W.; García, C. et al. Multidisciplinary Digital Publishing Institute MDPI (2022) CC
For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of Trypanosoma cruzi in human serum is affected by various influences including the choice of th... more
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Buruli ulcer laboratory network and new external quality assessment programme for PCR-based diagnosis in the WHO African Region. Terms of reference

World Health Organization WHO (2020) C_WHO
A total of 18 laboratories from 13 countries participated in the four rounds of EQA: 10 laboratories from eight African endemic countries, four of which participated in all four rounds and three in three rounds. The overall results showed that the median performance of these laboratories improved ov... more
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Durchführung des Nasopharynx- und Oropharynx-Abstrichs bei COVID-19-Diagnostik -- PCR-Test

AMBOSS (2021) CC
In diesem Video wird der korrekte Rachenabstrich bei der COVID-19 Diagnostik gezeigt: Einmal mit Zugang über die Nase und einmal über die Mundhöhle. Wir beziehen uns hierbei auf die Empfehlungen des RKI und der WHO. Aufgrund der aktuellen Situation verzichten wir im Folgenden auf die Verwendung v... more
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Lineamientos Para El Uso De Pruebas Moleculares RT-PCR Pruebas De Antígeno Y Pruebas Serológicas Para SARS-CoV-2 (COVID-19) en Colombia

Ministerio de Salud y Protección Social Bogotá, Colombia (2020) CC
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Collection of Dried Blood Spots (DBS) from Infants for PCR Testing

Centers for Disease Control and Prevention (CDC) (2018) C1
This clinical job aid provides health care workers with information on how to collect specimens for early infant diagnosis on dried blood spots, as well as drying and packaging for transport.
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Implementación y aplicación costo-efectiva del sistema cerrado de PCR en tiempo real (RT-PCR) Xpert-MTB/Rif avalado por OMS para la detección del complejo Mycobacterum tuberculosis y resistencia a ri

World Health Organization Americas; Pan American Health Organization (2017) C_WHO
Consenso de los Laboratorios Supranacionales (LSN), Centros Colaboradores (CC) y Laboratorios Nacionales de Referencia (LNR) de “países priorizados” para la aplicación del Xpert-MTB/Rif en Las Américas. Guatemala, 11-12 abril de 2011 Accessed December 2017
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India: Dried Blood Spots (DBS) Sample collection for Early Infant Diagnosis (EID) by PCR

National AIDS Control Organization (NACO) (2015) C1
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Diagnóstico de Vibrio cholerae y Salmonella spp. por PCR

Organización Mundial de la Salud OMS (2010) C_WHO
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Mandat Réseau de laboratoires et nouveau modèle de programme d’évaluation externe de la qualité des tests de diagnostic par PCR de l’ulcère de Buruli dans la Région africaine de l’OMS

Organisation Mondiale de la Santé OMS (2020) C1
Au total, 18 laboratoires de 13 pays ont participé aux quatre cycles d'AQE : 10 laboratoires de huit pays africains endémiques, dont quatre ont participé aux quatre cycles et trois à trois cycles. Les résultats globaux ont montré que la performance médiane de ces laboratoires s'est amélioré... more
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Chagas Disease: Detection of Trypanosoma cruzi by a New, High-Specific Real Time PCR

Kann, S.; Kunz, M.; Hansen, J.; et al. (2020) CC
J Clin Med . 2020 May 18;9(5):1517. doi: 10.3390/jcm9051517. Chagas disease (CD) is a major burden in Latin America, expanding also to non-endemic countries. A gold standard to detect the CD causing pathogen Trypanosoma cruzi is currently not available. Existing real time polymerase chain reaction... more
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Hinweise zur Testung von Patienten auf Infektion mit dem neuartigen Coronavirus SARS-CoV-2

Robert-Koch-Institut RKI (2021) C2
Probenmaterial für die PCR-Diagnostik zum direkten Erregernachweis Verpackung und Versand Empfehlungen zum Umgang mit Probenmaterial Direkter Erregernachweis durch RT-PCR Antikörpernachweise (indirekter Nachweis einer Infektion) Antigennachweise Bemerkungen zur Interp... more
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Report of a WHO–FIND meeting on diagnostics for Buruli ulcer

WHO Tecnical Advisory Group and FIND (2018) CC
The meeting was held from 26 to 27 March 2018 to review and discuss the following topics: Advances and challenges in the use of fTLC, and new approaches to detecting mycolactone using monoclonal antibodies (mAbs). The status of development of rapid diagnostic tests (RDTs) targeting the MUL... more