Neglected Tropical Diseases (NTDs) continue to cause hardship and harm to over one billion people worldwide, burdening individuals, families and communities who are already marginalized and disadvantaged. Taking a One Health approach that recognizes the relationship between human, animal, and environmental health is key to sustainably addressing NTDs. This course provides practical ideas, tools, and examples to enable each of us to take One Health action towards the global goal of substantially reducing the burden of NTDs by 2030.